More on Calcium Ion Activity Buffers for Coagulation Testing
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More on calcium ion activity buffers for coagulation testing.
terpreted as postovulatory luteal phase rather than follicular. Assuming a 2.2 mg/L positive bias with SST, our current laboratory follicular phase progesterone reference range of ,1.6 mg/L could increase to ,3.8 mg/L with SST. We recommend that all progesterone serum samples be collected in plain glass tubes until this SST interference problem is resolved. Our laboratory has notified Roche Dia...
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BACKGROUND Ca(2+) activity close to the physiological concentration of 1.3 mmol/L is essential in blood coagulation. Is this also true for the performance of global diagnostic coagulation assays? We searched for compounds that would buffer Ca(2+) activity at approximately 1.3 mmol/L without disturbing coagulation reactions and investigated whether such Ca(2+) buffering improves diagnostic effic...
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The production of hydrogen ions during the hydrolysis of ATP by myosin B and the consequent fall in pH can produce alterations in enzymatic activity directly, or by influencing Ca ion concentrations controlled by metal-chelate systems. Adequate hydrogen ion buffer systems can limit pH changes to acceptable levels. However, several commonly used hydrogen ion buffers have been reported to have sp...
متن کاملCalcium ion-dependent p-nitrophenyl phosphate phosphatase activity and calcium ion-dependent adenosine triphosphatase activity from human erythrocyte membranes.
In the presence of ATP and of Mg(2+), human erythrocyte membranes show a phosphatase activity towards p-nitrophenyl phosphate which is activated by low concentrations of Ca(2+). The effect of Ca(2+) is strongly enhanced if either K(+) or Na(+) is also present. Activation of the p-nitrophenyl phosphate phosphatase by Ca(2+) reaches a half-maximum at about 8mum-Ca(2+) and is apparent only when th...
متن کاملCointegration: More on Testing Methods
An alternative to the Engle-Granger tests is the Cointegration Regression Durbin-Watson (CRDW) test of Sargan and Bhargava (1983). The test involves a simple regression of one variable on the other, and the standard Durbin-Watson test on the residuals. Recall the null for the DW test is that the residuals form a nonstationary random walk, whereas the alternative hypothesis is that the residuals...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1999
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/45.9.1575